We investigated the ability of human vision to distinguish colors in order to help guide the guideline conceived for developing the display market. We made the following tests to measure human coloring ability; testers select 2 color tiles of different colors from a total of 16 color tiles, earned points when correctly matched, and measured the color separation ability for a specific color. We tested it on a web page to get more data. Based on the basic information of the participants, the color discrimination ability was measured using the CIE Lab color space for each gender and age, and the color discrimination ability of the 10th to 20th groups was the highest, and men have a better ability than women. We are planning to develop a program which makes suitable color contrast on display.